Description
Principle of the Assay: FASL ELISA kit applies the quantitative sandwich enzyme inimunoassay technique. The niicrotiter plate has been pre-coated with a monoclonal antibody specific for FASL. Standards or samples are then added to the niicrotiter plate wells and FASL if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of FASL present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for FASL are added to each well to "sandwich" the FASL immobilized on the plate. The niicrotiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain FASL and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FASL concentration in each sample is interpolated from this standard curve